KMID : 0545120170270010197
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Journal of Microbiology and Biotechnology 2017 Volume.27 No. 1 p.197 ~ p.205
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Pharmacologic Inhibition of Autophagy Sensitizes Human Acute Leukemia Jurkat T Cells to Acacetin-Induced Apoptosis
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Lee Ji-Young
Jun Do-Youn Kim Ki-Yun Ha Eun-Ji Woo Mi-Hee Ko Jee-Youn Yun Young-Ho Oh In-Seok Kim Young-Ho
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Abstract
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Exposure of Jurkat T cell clone (J/Neo cells) to acacetin (5,7-dihydroxy-4¡¯-methoxyflavone), which is present in barnyard millet (Echinochloa esculenta (A. Braun)) grains, caused cytotoxicity, enhancement of apoptotic sub-G1 rate, Bak activation, loss of mitochondrial membrane potential (¥Ä¥÷m), activation of caspase-9 and caspase-3, degradation of poly(ADPribose) polymerase, and FITC-Annexin V-stainable phosphatidylserine exposure on the external surface of the cytoplasmic membrane without accompanying necrosis. These apoptotic responses were abrogated in Jurkat T cell clone (J/Bcl-xL) overexpressing Bcl-xL. Under the same conditions, cellular autophagic responses, including suppression of the AktmTOR pathway and p62/SQSTM1 down-regulation, were commonly detected in J/Neo and J/Bcl-xL cells; however, formation of acridine orange-stainable acidic vascular organelles, LC3-I/II conversion, and Beclin-1 phosphorylation (Ser-15) were detected only in J/Neo cells. Correspondingly, concomitant treatment with the autophagy inhibitor (3-methyladenine or LY294002) appeared to enhance acacetin-induced apoptotic responses, such as Bak activation, ¥Ä¥÷m loss, activation of caspase-9 and caspase-3, and apoptotic sub-G1 accumulation. This indicated that acacetin could induce apoptosis and cytoprotective autophagy in Jurkat T cells simultaneously. Together, these results demonstrate that acacetin induces not only apoptotic cell death via activation of Bak, loss of ¥Ä¥÷m, and activation of the mitochondrial caspase cascade, but also cytoprotective autophagy resulting from suppression of the Akt-mTOR pathway. Furthermore, pharmacologic inhibition of the autophagy pathway augments the activation of Bak and resultant mitochondrial damage-mediated apoptosis in Jurkat T cells.
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KEYWORD
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Acacetin, Bak activation, cytoprotective autophagy, Echinochloa esculenta (A. Braun), Jurkat T cells, mitochondria-dependent apoptosis
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